Gotham River is the river that separates Gotham City with the rest of Gotham County. It connects to the Atlantic Ocean and provides the basis for fishing and cargo business in the Gotham Docklands. Various bridges are needed to connect the city in itself, as well as with the mainland, particulary a bridge at the city's west coast leading to the Gotham Woods. The Arkham District island, the Tricorner, the Narrows island, the Blackgate isle and others are located in the Gotham River, while various old steam tunnels exist under the riverbed.
When Oswald Cobblepot was exposed as the traitor who snitched to the Major Crimes Unit, Carmine Falcone assigned James Gordon to dispose of Penguin. As soon as they arrived at the Gotham Harbor, Harvey Bullock opened the trunk, revealing a whimpering Oswald Cobblepot inside. Bullock explained Gordon that Falcone had ordered him to kill Cobblepot in order to show that he will remain silent about the frame-up. At first Jim refused, but Bullock revealed that if he doesn't kill the traitor, then Harvey has orders to kill both of them. Admiting that he likes Gordon, Bullock said that he won't find it easy to kill him, but he will try, because the only alternative is that Falcone will kill all three of them, and likely Barbara Kean as well. Feeling he has no choice, Gordon drew his gun and marched Cobblepot to the end of the pier. On the way, Cobblepot warned Gordon that a terrible war is coming, as Falcone's enemies will try to take advantage of his perceived weakness; Cobblepot pleaded that he can be a useful spy for Gordon, and help prevent the bloodshed that will surely come, but Gordon told him to shut up and spined him around to face the water. Oswald pleaded for mercy as Gordon placed the gun to the back of his head - then leaned in and warned him to never come back to Gotham, before firing the gun past his ear and shoving him into the Gotham River. From where Bullock is standing, it looks as though Gordon has done his job. Once the cops were gone, Penguin resurfaced from the Gotham River and reached the land. However, Cobblepot then proceeded to murder a fisherman for his sandwich in a crazed rage of hunger. He then looked towards the other side of the Gotham River, starting to think about his further actions.
- The Gotham River is a recurring location in the DC comics.